KMID : 0608520080140010129
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Korean Journal of Oriental Medicine 2008 Volume.14 No. 1 p.129 ~ p.135
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Geographical variation and evolutionary relationship of Asparagus cochinchinensis Lour. based on rDNA-ITS sequences and Random Amplified Polymorphic DNA(RAPD)
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Moon Byeong-cheol
Choo Byung-kil Ji Yun-Ui Choi Go-Ya Yoon Tae-sook Lee A-eong Kim Ho-kyoung
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Abstract
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Phylogenetic relationship and DNA polymorphism among local populations of the Asparagus cochinchinensis have been investigated based on nuclear ribosomal DNA ITS sequences and RAPD analysis in Korea. In result, two genetically distinct groups of local populations except Geoje were recognized by the phylogenetic tree both in rDNA-ITS and RAPD. One was called ¡®western coast group¡¯ that includes the Buan 1, 2 and Taean and the other was ¡®southern coast group¡¯ that includes Haenam, Yeosu and Namhae. Thus, the geographical relationship of Asparagus cochinchinensis was two well-typified clades. These results suggest that the geographical genetic variation of Asparagus cochinchinensis is closely connected with the slow and long period of propagation via the coast in Korean Peninsula.
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KEYWORD
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Asparagus cochinchinensis, internal transcribed spacer(ITS), random amplified polymorphic DNA(RAPD), genetic variation, geographical population
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