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KMID : 0608520080140010129
Korean Journal of Oriental Medicine
2008 Volume.14 No. 1 p.129 ~ p.135
Geographical variation and evolutionary relationship of Asparagus cochinchinensis Lour. based on rDNA-ITS sequences and Random Amplified Polymorphic DNA(RAPD)
Moon Byeong-cheol

Choo Byung-kil
Ji Yun-Ui
Choi Go-Ya
Yoon Tae-sook
Lee A-eong
Kim Ho-kyoung
Abstract
Phylogenetic relationship and DNA polymorphism among local populations of the Asparagus cochinchinensis have been investigated based on nuclear ribosomal DNA ITS sequences and RAPD analysis in Korea. In result, two genetically distinct groups of local populations except Geoje were recognized by the phylogenetic tree both in rDNA-ITS and RAPD. One was called ¡®western coast group¡¯ that includes the Buan 1, 2 and Taean and the other was ¡®southern coast group¡¯ that includes Haenam, Yeosu and Namhae. Thus, the geographical relationship of Asparagus cochinchinensis was two well-typified clades. These results suggest that the geographical genetic variation of Asparagus cochinchinensis is closely connected with the slow and long period of propagation via the coast in Korean Peninsula.
KEYWORD
Asparagus cochinchinensis, internal transcribed spacer(ITS), random amplified polymorphic DNA(RAPD), genetic variation, geographical population
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